1JN9
Structure of Putative Asparaginase Encoded by Escherichia coli ybiK Gene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-22 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.941, 70.425, 148.862 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.1702 |
| Rwork | 0.170 |
| R-free | 0.23248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | structure of a different crystal form available in the laboratory |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.310 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.064 | 0.222 |
| Number of reflections | 29401 | |
| <I/σ(I)> | 19.8 | 5.3 |
| Completeness [%] | 95.4 | |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 273 | Tris HCl pH 8.5, 0.2 M calcium chloride, PEG 4000, PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.2 (M) | ||
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 3 | 1 | reservoir | PEG400 | 15 (%) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) |
