1JMU
Crystal Structure of the Reovirus mu1/sigma3 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-25 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 180.294, 184.942, 284.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24100 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.116 | 0.346 |
| Number of reflections | 107661 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 94.6 | 57.3 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium sulfate, n-octyl-b-D-glucopyranoside, DTT, Pipes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dithiothreitol | 10 (mM) | |
| 2 | 1 | reservoir | PIPES-NaOH | 100 (mM) | pH7.0 |
| 3 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | n-octyl-beta-D-glucopyranoside | 0.07 (%(w/v)) |
