1JLW
Anopheles dirus species B glutathione S-transferases 1-4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2000-04-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5405 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 49.440, 49.440, 271.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.920 - 2.450 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Lucilia cuprina GST |
RMSD bond length | 0.007 |
RMSD bond angle | 21.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 14237 | |
<I/σ(I)> | 13.1 | 2.2 |
Completeness [%] | 93.9 | 83.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 298 | PEG 8000, glutathione, cacodylate, glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium acetate | 0.2 (M) | |
2 | 1 | reservoir | PEG8000 | 30 (%) | |
3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |