1JLK
Crystal structure of the Mn(2+)-bound form of response regulator Rcp1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.511, 78.561, 43.710 |
| Unit cell angles | 90.00, 107.68, 90.00 |
Refinement procedure
| Resolution | 14.700 - 2.300 |
| Rwork | 0.225 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i3c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.047 | 0.096 |
| Number of reflections | 10745 | |
| <I/σ(I)> | 29.1 | 12.6 |
| Completeness [%] | 92.0 | 78.8 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
