1JL2

Crystal structure of TCEO RNase H-a chimera combining the folding core from T. thermophilus RNase H and the remaining region of E. coli RNase H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-28
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	56.195, 87.033, 60.687
Unit cell angles	90.00, 91.87, 90.00

Refinement procedure

Resolution	20.000 - 1.760
R-factor	0.249 *
Rwork	0.249
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f21
RMSD bond length	0.005 *
RMSD bond angle	1.250 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.830
High resolution limit [Å]	1.760	1.760
Rmerge	0.061	0.277
Total number of observations	62607 *
Number of reflections	5255 *	189 *
Completeness [%]	97.7	95.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	50 mM Tris, pH 8.0, 18% PEG600, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	Tris	50 (mM)	pH8.0
3	1	reservoir	PEG600	18 (%)