1JL1

D10A E. coli ribonuclease HI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-28
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	37.016, 40.874, 85.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.300
R-factor	0.223 *
Rwork	0.223
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f21
RMSD bond length	0.008 *
RMSD bond angle	2.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.360
High resolution limit [Å]	1.300	1.300
Rmerge	0.088	0.188 *
Number of reflections	28054
Completeness [%]	99.4	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	20 (mM)	pH8.0
2	1	reservoir	PEG3350	10 (%(w/v))
3	1	drop	protein	6 (mg/ml)