1JJA
CRYSTAL STRUCTURE OF ORTHORHOMBIC FORM OF D90E MUTANT OF ESCHERICHIA COLI L-ASPARAGINASE II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 225.419, 128.049, 62.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.20416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Active dimer of native E.coli asparaginase II (PDB code: 3ECA) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.410 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.123 | 0.813 |
| Number of reflections | 75961 | |
| <I/σ(I)> | 11.2 | 1.8 |
| Completeness [%] | 93.9 | |
| Redundancy | 3.14 | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 292 | PEG MME550, bicine, NaCl , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
