1JIW

Crystal structure of the APR-APRin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-10-11
Detector	MARRESEARCH
Wavelength(s)	0.873
Spacegroup name	P 21 21 2
Unit cell lengths	75.627, 118.432, 91.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.930 - 1.740
R-factor	0.184 *
Rwork	0.184
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1akl
RMSD bond length	0.004
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.930	1.770
High resolution limit [Å]	1.740	1.740
Rmerge	0.037 *	0.212 *
Total number of observations	310401 *
Number of reflections	84597
Completeness [%]	98.5	78.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.6	20 *	tri-Na-citrate, isopropanol, PEG 4000, pH 5.6, VAPOR DIFFUSION, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%)
2	1	reservoir	isopropanol	20 (%)
3	1	reservoir	citrate	0.1 (M)	pH5.6