1JHN
Crystal Structure of the Lumenal Domain of Calnexin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.772, 85.772, 143.476 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.800 - 2.900 |
| R-factor | 0.327 * |
| Rwork | 0.327 |
| R-free | 0.37700 |
| Structure solution method | MIRAS, SAS, MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.046 | 0.245 |
| Total number of observations | 82347 * | |
| Number of reflections | 12339 | |
| <I/σ(I)> | 23.4 | 5.4 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | used microseeding, Hahn, M., (1998) J. Struct. Biol., 123, 260. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | MPD | 10 (%) | |
| 3 | 1 | drop | HEPES | 20 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | sucrose | 2 (%) | |
| 6 | 1 | reservoir | MPD | 25 (%) | |
| 7 | 1 | reservoir | PEG8000 | 10 (%) | |
| 8 | 1 | reservoir | HEPES | 150 (mM) | |
| 9 | 1 | reservoir | 5 (mM) |
