1JHJ

Crystal structure of the APC10/Doc1 subunit of the human anaphase-promoting complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-18
Detector	MARRESEARCH
Wavelength(s)	0.9791, 0.9797, 0.9500, 1.4800, 1.600
Spacegroup name	P 6 2 2
Unit cell lengths	97.664, 97.664, 67.769
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 * - 1.600
R-factor	0.209 *
Rwork	0.209
R-free	0.24400
Structure solution method	MAD
RMSD bond length	0.012 *
RMSD bond angle	1.711 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	1.600	1.600
Rmerge	0.047
<I/σ(I)>	35.86
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	18 *	ammonium sulfate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.9 (M)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir		0.1 (M)
4	1	reservoir	nickel acetate	5 (mM)
5	1	reservoir	aniline	5 (%)