1JH9
PURINE REPRESSOR MUTANT-HYPOXANTHINE-PURF OPERATOR COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 175.380, 92.610, 80.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.550 |
| R-factor | 0.219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pnr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.610 | |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.112 | 0.524 |
| Number of reflections | 22093 | |
| <I/σ(I)> | 4.89 | 1.4 |
| Completeness [%] | 99.8 | 100 * |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, ammonium sulfate, ammonium phosphate, cobalt hexammine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | NH4SO4 | ||
| 3 | 1 | 1 | NH4PO4 | ||
| 4 | 1 | 1 | Co(NH3)6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 25 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
| 4 | 1 | reservoir | cobalt hexammine chloride | 50 (mM) | |
| 5 | 1 | reservoir | ammonium phosphate | 0.1 (M) | pH7.5 |
