1JGY

Photosynthetic Reaction Center Mutant With Tyr M 76 Replaced With Phe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-04-11
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 31 2 1
Unit cell lengths	141.680, 141.680, 186.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.100 - 2.700
R-factor	0.212 *
Rwork	0.218
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qov
RMSD bond length	0.008
RMSD bond angle	1.310 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.103	0.210
Total number of observations	75641 *
Number of reflections	35678
Completeness [%]	59.5	38.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	298	Potasium phosphate, LDAO, heptane triol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	LDAO	0.08 (%)
3	1	drop	potassium phosphate	0.75 (M)
4	1	drop	heptanetriol	3.5 (%)
5	1	reservoir	potassium phosphate	1.6 (M)