1JEK

Visna TM CORE STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-12-01
Detector	RIGAKU RAXIS IV
Spacegroup name	P 3 2 1
Unit cell lengths	52.548, 52.548, 61.686
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.960 - 1.500
R-factor	0.213
Rwork	0.213
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2siv
RMSD bond length	0.012
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.520
High resolution limit [Å]	1.500	1.500
Rmerge	0.066	0.259 *
Total number of observations	52353 *
Number of reflections	16300
<I/σ(I)>	13.9	2.5
Completeness [%]	90.1	64.7
Redundancy	7.5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	25% tert-butanol, 0.1M TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	tert-butanol	25 (%)
3	1	reservoir	Tris-HCl	0.1 (M)