1JE6

Structure of the MHC Class I Homolog MICB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.10, 0.97953, 0.9793, 0.9567
Spacegroup name	P 4 21 2
Unit cell lengths	81.700, 81.700, 86.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.2588
Rwork	0.255
R-free	0.29800 *
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	25.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.620	0.353
Number of reflections	10656
<I/σ(I)>	49.9	6.8
Completeness [%]	99.6	100
Redundancy	10.1	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	22 *	ammonium sulfate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	reservoir	ammonium sulfate	1.2 (M)
3	1	reservoir	Tris	50 (mM)	pH8.5