1JDL

Structure of cytochrome c2 from Rhodospirillum Centenum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200HB
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1999-10-10
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	29.700, 59.900, 65.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.192 *
Rwork	0.190
R-free	0.21000
Structure solution method	SAD, MIR,MOLECULAR REPLACEMENT
Starting model (for MR)	2c2c
RMSD bond length	0.005 *
RMSD bond angle	1.330 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	20.000
High resolution limit [Å]	1.700	1.700
Rmerge	0.070	0.320
Total number of observations	223519 *
Number of reflections	13248	1169 *
<I/σ(I)>	2986	149
Completeness [%]	98.6	88.4
Redundancy	16.9	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277 *	PEG 6000, potassium phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	277 *	PEG 6000, potassium phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	277 *	PEG 6000, potassium phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG6000	10 (%)
3	1	reservoir	phosphate	0.1 (M)	pH7