1JDF
Glucarate Dehydratase from E.coli N341D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 1999-12-01 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.925, 83.926, 98.222 |
| Unit cell angles | 104.11, 93.75, 113.12 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ec8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.455 |
| Total number of observations | 505535 * | |
| Number of reflections | 132888 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 97.1 | 89 * |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 8 | 4 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 10 (mM) | |
| 2 | 1 | 1 | 5 (mM) | ||
| 3 | 1 | 1 | protein | 13.3 (mg/ml) | |
| 4 | 1 | 2 | mPEG5000 | 14 (%) | |
| 5 | 1 | 2 | 50 (mM) | ||
| 6 | 1 | 2 | 2-propanol | 5 (%) | pH8.0 |
| 7 | 1 | 2 | HEPPS | 5 (%) |
