1JD4
Crystal Structure of DIAP1-BIR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-30 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | I 4 |
| Unit cell lengths | 96.100, 96.100, 59.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.236 * |
| Rwork | 0.236 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g73 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.334 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.113 | 0.437 |
| Total number of observations | 36725 * | |
| Number of reflections | 7220 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 95.1 | 95.5 |
| Redundancy | 5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | Tris, 2,4-methyl-pentanediol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
| 3 | 1 | reservoir | MPD | 60 (%(v/v)) | |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) |
