1JCE
MREB FROM THERMOTOGA MARITIMA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9780, 0.9802, 0.9394 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.630, 70.130, 82.180 |
| Unit cell angles | 90.00, 108.79, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.1973 |
| Rwork | 0.197 |
| R-free | 0.23360 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.233 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.063 | 0.239 |
| Number of reflections | 22453 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 93.1 | 67.5 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 19 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | isopropanol | 10 (%) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | HEPES | 0.1 (M) | |
| 4 | 1 | drop | protein | 8 (mg/ml) |
