1JC7

The Laminin-Binding Domain of Agrin is Structurally Related to N-TIMP-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	0.847
Spacegroup name	C 1 2 1
Unit cell lengths	85.306, 49.899, 54.328
Unit cell angles	90.00, 118.43, 90.00

Refinement procedure

Resolution	27.920 - 2.730
R-factor	0.207
Rwork	0.207
R-free	0.24900
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.700 *
Rmerge	0.086 *	0.272 *
Completeness [%]	99.1 *	94.3 *
Redundancy	4.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	4 *	PEG4000, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	citrate	0.1 (M)
3	1	reservoir	citrate	0.1 (M)
4	1	reservoir	PEG4000	20 (%(w/v))
5	1	reservoir	2-propanol	10 (%(w/v))
6	1	reservoir	MPD	10 (%(w/v))