1JBO
The 1.45A Three-Dimensional Structure of c-Phycocyanin from the Thermophylic Cyanobacterium Synechococcus elongatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.244 |
Spacegroup name | H 3 2 |
Unit cell lengths | 187.995, 187.995, 60.542 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 95.000 * - 1.450 |
Rwork | 0.146 |
R-free | 0.18800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cpc |
RMSD bond length | 0.039 |
RMSD bond angle | 4.632 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.000 * | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.088 | 0.261 |
Total number of observations | 485268 * | |
Number of reflections | 71757 | 10410 * |
<I/σ(I)> | 5.7 | 2.5 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 20 * | Ammonium Sulphate, MES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.1 (M) | |
2 | 1 | reservoir | MES | 40 (mM) | pH6.1 |
3 | 1 | drop | protein | 20 (mg/ml) |