1JBG
Crystal Structure of MtaN, the Bacillus subtilis Multidrug Transporter Activator, N-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 49.382, 67.838, 115.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.750 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.28670 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.300 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.072 | 0.271 |
| Total number of observations | 42297 * | |
| Number of reflections | 5199 | |
| <I/σ(I)> | 7.9 | 2.7 |
| Completeness [%] | 99.3 * | 99 |
| Redundancy | 8.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Godsey, M., (2000) Acta Crystallogr., D56, 1456. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | 3.77 (M) | ||
| 3 | 1 | reservoir | HEPES | 50 (mM) | pH7.0 |
| 4 | 1 | reservoir | 10 (mM) |
