1JB9

Crystal Structure of The Ferredoxin:NADP+ Reductase From Maize Root AT 1.7 Angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-03-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	59.670, 59.670, 189.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 1.700
R-factor	0.164 *
Rwork	0.164
R-free	0.22300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Modified 1FNB
RMSD bond length	0.013
RMSD bond angle	17.910 *
Data scaling software	SCALEPACK
Phasing software	MRX
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	1.700 *	1.700
Rmerge	0.068 *	0.364 *
Total number of observations	246386 *
Number of reflections	43654 *
<I/σ(I)>	13.5	3
Completeness [%]	99.1 *	97.6 *
Redundancy	5.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	298	Peg 8000, Na Cacodylate, Mg Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	HEPES	10 (mM)	pH7.0
3	1	reservoir	PEG8000	20 (%)
4	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5
5	1	reservoir	magnesium acetate	0.2 (M)