1JB7
DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.100, 93.100, 421.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.860 |
| R-factor | 0.23 * |
| Rwork | 0.230 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1otc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.065 | 0.540 |
| Total number of observations | 903239 * | |
| Number of reflections | 92060 | |
| <I/σ(I)> | 16.9 | 2.2 |
| Completeness [%] | 99.1 | 95.6 |
| Redundancy | 9.8 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 282 | drop contains the same amount of reservoir solution except 50mM NaCl * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | ethylene glycol | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | MES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | alpha subunit | 0.2 (M) | |
| 10 | 1 | reservoir | PEG4000 | 3-8 (%) | |
| 11 | 1 | reservoir | MES | 40 (mM) | |
| 12 | 1 | reservoir | 0.02 (%) | ||
| 13 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 14 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | drop | beta subunit | 1.0-1.5 equivalents each of | |
| 3 | 1 | drop | ssDNA | ||
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | Tris-HCl | 5 (mM) | |
| 6 | 1 | drop | EDTA | 0.1 (mM) | |
| 7 | 1 | drop | 0.02 (%) | ||
| 8 | 1 | drop | dithiothreitol | 2 (mM) | |
| 9 | 1 | reservoir | ethylene glycol | 30 (%) |
