1JB6

Crystal Structure of Dimerization Domain (1-33) of HNF-1alpha

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-24
Detector	SBC-2
Wavelength(s)	0.9794, 0.9800, 1.0030
Spacegroup name	P 21 21 2
Unit cell lengths	28.420, 42.190, 42.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.700
R-factor	0.231 *
Rwork	0.231
R-free	0.24500
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.800
Data reduction software	d*TREK ((D*TREK))
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	5245
Completeness [%]	88.0
Redundancy	6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	4 *	MPD, Tris-HCl, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	3 (mM)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	dithiothreitol	2 (mM)
4	1	reservoir	MPD	20 (%(v/v))
5	1	reservoir	Tris-HCl	50 (mM)
6	1	reservoir	dithiothreitol	1 (mM)