1JAI

H-RAS P21 PROTEIN MUTANT G12P, COMPLEXED WITH GUANOSINE-5'-[BETA,GAMMA-METHYLENE] TRIPHOSPHATE AND MANGANESE

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	1992-11-16
Detector	SIEMENS
Spacegroup name	P 32 2 1
Unit cell lengths	40.000, 40.000, 160.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.2
Rwork	0.200
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	C-H-RAS:GPPNP:MG
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	XDS ((W.KABSCH))
Data scaling software	XSCALE ((KABSCH))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.061 *
Total number of observations	56489 *
Number of reflections	13392
<I/σ(I)>	22.2	4.3
Completeness [%]	92.0	71
Redundancy	4.2	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	DTE	20 (mM)
2	1	1	PEG400	25-30 (%(w/v))
3	1	1		2.5 (mM)
4	1	1	protein	10 (mg/ml)