1JAD

C-terminal Domain of Turkey PLC-beta

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97949, 0.97940, 0.97526
Spacegroup name	P 1
Unit cell lengths	39.111, 51.928, 79.350
Unit cell angles	101.07, 96.96, 100.70

Refinement procedure

Resolution	20.000 * - 2.400
R-factor	0.254 *
Rwork	0.254
R-free	0.29300 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	14.800 *
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.079	0.171
Number of reflections	20867
<I/σ(I)>	12.6	6.2
Completeness [%]	91.8	75.1
Redundancy	5.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	PEG 2000, bis-tris, lithium sulfate, glycerol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG2000	12-16 (%(w/v))
2	1	reservoir	Bis-Tris	80 (mM)	pH5.7-6.3
3	1	reservoir	ammonium sulfate	60-120 (mM)
4	1	reservoir	glycerol	10 (%(v/v))