1JAC
A NOVEL MODE OF CARBOHYDRATE RECOGNITION IN JACALIN, A MORACEAE PLANT LECTIN WITH A BETA-PRISM
Experimental procedure
| Source type | SEALED TUBE |
| Detector technology | AREA DETECTOR |
| Collection date | 1992 |
| Detector | SIEMENS |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 129.600, 129.600, 157.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.430 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.26000 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 27.800 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.430 * |
| Rmerge | 0.080 |
| Total number of observations | 219402 * |
| Number of reflections | 23791 |
| Completeness [%] | 78.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 10 | 1 | reservoir | 0.05 (%(w/v)) | ||
| 11 | 1 | reservoir | methyl-alpha-D-galactopyranoside | 60 (M) | |
| 2 | 1 | drop | phosphate | 0.02 (M) | |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | drop | 0.05 (%(w/v)) | ||
| 5 | 1 | drop | methyl-alpha-D-galactopyranoside | 60 (M) | |
| 6 | 1 | drop | PEG4000 | 5-6 (%(w/v)) | |
| 7 | 1 | reservoir | PEG4000 | 40 (%) | |
| 8 | 1 | reservoir | phosphate | 0.02 (M) | |
| 9 | 1 | reservoir | 0.1 (M) |
