1J9Y

Crystal structure of mannanase 26A from Pseudomonas cellulosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-01
Detector	MARRESEARCH
Wavelength(s)	0.8374
Spacegroup name	P 41
Unit cell lengths	93.240, 93.240, 54.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.500 - 1.850
R-factor	0.182 *
Rwork	0.182
R-free	0.20000
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	0.029
Data reduction software	DENZO (/ SCALEPACK)
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.850
High resolution limit [Å]	1.850	1.800
Rmerge	0.033	0.077
Number of reflections	40381
<I/σ(I)>	29.9	10.2
Completeness [%]	99.8	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG MME 550, zinc sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG550 MME	26 (%)
2	1	reservoir	zinc sulfate	0.012 (M)
3	1	reservoir	MES	0.1 (M)