1J9A

OLIGORIBONUCLEASE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	115
Detector technology	CCD
Collection date	2000-02-27
Detector	BRUKER
Wavelength(s)	0.9794, 0.9796, 0.9641, 0.9832
Spacegroup name	P 41 2 2
Unit cell lengths	55.110, 55.110, 144.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.1851
R-free	0.29860
RMSD bond length	0.006
RMSD bond angle	0.026
Data reduction software	HKL-2000
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.040	0.150
Number of reflections	14507
<I/σ(I)>	15	2
Completeness [%]	100.0	100
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.3		Protein solution: 15 mg/mL protein in 0.01 M HEPES, 0.15 M sodium chloride, 0.005 M DTT, 0.005 M EDTA with pH 7.1-8.0. Reservoir: 2.3 M ammonium sulfate at pH 7.1-7.5. Hanging drop vapor diffusion method. Drops were formed by combining 4 microliters reservoir solution with 4 microliters protein solution. Room temperature., pH 7.3