1J8M

Signal Recognition Particle conserved GTPase domain from A. ambivalens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	64.866, 128.020, 72.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.000
R-factor	0.21916
Rwork	0.216
R-free	0.27002
RMSD bond length	0.011 *
RMSD bond angle	1.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.000
Rmerge	0.051 *
Total number of observations	451808 *
Number of reflections	20842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	acetate	100 (mM)
3	1	reservoir	calcium acetate	100 (mM)
4	1	reservoir	PEG8000	18 (%)