1J8H
Crystal Structure of a Complex of a Human alpha/beta-T cell Receptor, Influenza HA Antigen Peptide, and MHC Class II Molecule, HLA-DR4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-08-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.753, 73.317, 123.033 |
Unit cell angles | 90.00, 108.52, 90.00 |
Refinement procedure
Resolution | 19.810 - 2.400 |
R-factor | 0.208 * |
Rwork | 0.211 |
R-free | 0.24500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1FYT.pdb |
RMSD bond length | 0.007 |
RMSD bond angle | 1.380 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.058 | 0.330 |
Number of reflections | 46705 * | |
<I/σ(I)> | 20.6 | |
Completeness [%] | 96.8 | 96.37 * |
Redundancy | 4.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 18 * | 13% PEG 8000, 1 M NaCl, 100 mM HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 13 (%) | |
3 | 1 | reservoir | 1 (M) | ||
4 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |