1J7X

CRYSTAL STRUCTURE OF A FUNCTIONAL UNIT OF INTERPHOTORECEPTOR RETINOID-BINDING PROTEIN (IRBP)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9777,0.9790,0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	63.144, 65.756, 68.174
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.800
R-factor	0.206 *
Rwork	0.205
R-free	0.23000 *
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	1.234 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (V1.17)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.250	1.846
High resolution limit [Å]	1.800	1.800
Rmerge	0.065	0.495
Number of reflections	26579
<I/σ(I)>	8.4
Completeness [%]	99.9	99.8
Redundancy	4.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	PEG 8000, TRIS, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	PEG 8000, TRIS, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	100 (mM)	pH8.5
2	1	reservoir		200 (mM)
3	1	reservoir	PEG8000	20 (%)