1J7T
Complex between Paromomycin and the 16S-rRNA A-site at 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.000, 45.900, 95.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 2.500 |
| R-factor | 0.206 * |
| Rwork | 0.206 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model containing the 16S rRNA A site as solved in the crystallographic structure of the 30S ribosomal particle complexed to paromomycin |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.055 * | 0.230 * |
| Number of reflections | 5278 * | |
| <I/σ(I)> | 24.4 | 6.5 |
| Completeness [%] | 95.0 | 97.4 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 21 * | MPD, NaCl, MgSO4, glycerol, Na cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | MgSO4 | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | sodium cacodylate | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.75-2.5 (%) | ||
| 2 | 1 | drop | glycerol | 0.5-1 (%) | |
| 3 | 1 | drop | paromomycin | 2 (mM) | |
| 4 | 1 | drop | 56.25-131.25 (mM) | ||
| 5 | 1 | drop | 2.5-7.5 (mM) | ||
| 6 | 1 | drop | sodium cacodylate | 62.5 (mM) | pH6.4 |
| 7 | 1 | drop | RNA | 0.5 (mM) | |
| 8 | 1 | reservoir | MPD | 40-60 (%) |
