1J73

Crystal structure of an unstable insulin analog with native activity.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-26
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	79.220, 79.220, 36.884
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.205 *
Rwork	0.205
R-free	0.27300
RMSD bond length	0.015 *
RMSD bond angle	2.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.056 *	0.097
Number of reflections	5448
Completeness [%]	93.0 *	100
Redundancy	3	611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8 *	298	Tris, phonel, acetone, sodium citrate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		0.02 (M)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir	sodium citrate	0.1 (M)	pH7.8
5	1	reservoir	zinc acetate	0.02 (%)
6	1	reservoir	acetate	8 (%)
7	1	reservoir	phenol	0.5 (%)