1J6Z
UNCOMPLEXED ACTIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.808, 37.498, 85.261 |
| Unit cell angles | 90.00, 108.26, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.540 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ATN.PDB |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.000 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.000 | 1.610 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.061 | 0.308 |
| Number of reflections | 48139 | |
| <I/σ(I)> | 23.3 | |
| Completeness [%] | 97.0 * | 94.2 |
| Redundancy | 7.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 2000 MONOMETHYLETHER 22%, CALCIUM ACETATE 200mM, TRIS 10mM (pH 7), pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 2000 MONOMETHYLETHER 22%, CALCIUM ACETATE 200mM, TRIS 10mM (pH 7), pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 MME | 22 (%) | |
| 3 | 1 | reservoir | Tris | 10 (mM) | pH7.0 |
| 4 | 1 | reservoir | calcium acetate | 200 (mM) |
