1J5U

CRYSTAL STRUCTURE OF AN ARCHEASE, POSSIBLE CHAPERONE (TM1083) FROM THERMOTOGA MARITIMA AT 2.0 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.925223, 0.978645, 0.979029
Spacegroup name	P 43 3 2
Unit cell lengths	105.280, 105.280, 105.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.090 - 2.000
R-factor	0.212
Rwork	0.212
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.540
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.176	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	13851
<I/σ(I)>	16.8	4.9
Completeness [%]	99.3	98.4
Redundancy	5.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	293	0.14 M CaCl2; 14 % isoprop; 0.07 M Acetate pH 4.6, 30 % Glycerol, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K, pH 4.60