1J4N
Crystal Structure of the AQP1 water channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Detector | ADSC |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 93.331, 93.331, 180.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.200 |
R-factor | 0.266 * |
Rwork | 0.266 |
R-free | 0.30800 |
RMSD bond length | 0.006 |
RMSD bond angle | 19.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.062 | 0.582 * |
Number of reflections | 19626 | |
<I/σ(I)> | 41.8 | |
Completeness [%] | 99.7 * | 100 * |
Redundancy | 15.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 | 277 | Sui, H., (2000) Acta Crystallogr., D56, 1198. * |
1 | Vapor diffusion, sitting drop * | 7.5 | 277 | Sui, H., (2000) Acta Crystallogr., D56, 1198. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
3 | 1 | reservoir | PEG550 MME | 20 (%) | |
4 | 1 | reservoir | Tris-HCl | 10 (mM) | pH7.5 |