1J49
INSIGHTS INTO DOMAIN CLOSURE, SUBSTRATE SPECIFICITY AND CATALYSIS OF D-LACTATE DEHYDROGENASE FROM LACTOBACILLUS BULGARICUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1994-12-01 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.400, 79.400, 228.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.200 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dld |
RMSD bond length | 0.015 |
RMSD bond angle | 0.025 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.071 | 0.210 |
Number of reflections | 38147 | |
<I/σ(I)> | 19.4 | 3.73 |
Completeness [%] | 98.7 | 93.3 |
Redundancy | 5.8 | 6.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 | pH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG2000 Me | 25 (%) | |
2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
3 | 1 | reservoir | NADH | 5 (mM) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |