1J3H

Crystal structure of apoenzyme cAMP-dependent protein kinase catalytic subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	97
Detector technology	IMAGE PLATE
Collection date	1999-01-04
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 1 21 1
Unit cell lengths	48.776, 143.601, 62.809
Unit cell angles	90.00, 105.71, 90.00

Refinement procedure

Resolution	19.960 - 2.900
Rwork	0.257
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctp
RMSD bond length	0.010 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	2.900
High resolution limit [Å]	2.900 *	2.800
Rmerge	0.055	0.493 *
Total number of observations	102700 *
Number of reflections	18800 *
Completeness [%]	91.8	82.8
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	Tris-hydrogen chloride, MPD, Bicine, ammonium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (mM)	pH7.5
2	1	reservoir	MPD	10-20 (%(v/v))
3	1	drop	MPD	3 (%(v/v))
4	1	drop	Bicine	0.1 (mM)	pH8.0
5	1	drop	protein	7.5 (mg/ml)