1J30

The crystal structure of sulerythrin, a rubrerythrin-like protein from a strictly aerobic and thermoacidiphilic archaeon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-23
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 63
Unit cell lengths	72.428, 72.428, 98.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.290 - 1.700
R-factor	0.192
Rwork	0.192
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ryt
RMSD bond length	0.005
RMSD bond angle	17.000 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.250	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.054	0.346
Total number of observations	171729 *
Number of reflections	32087
<I/σ(I)>	10.1	2.2
Completeness [%]	99.8	99.3
Redundancy	5.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	ammonium sulfate, HEPES-NaOH, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)	pH8.0
3	1	reservoir	ammonium sulfate	2.0 (M)
4	1	reservoir	HEPES-NaOH	10 (mM)	pH7.5