1J2B
Crystal Structure Of Archaeosine tRNA-Guanine Transglycosylase Complexed With lambda-form tRNA(Val)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 30 |
Detector technology | CCD |
Collection date | 2002-06-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 230.834, 230.834, 269.255 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 * - 3.300 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iq8 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.570 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.099 | 0.487 |
Total number of observations | 334265 * | |
Number of reflections | 41049 * | |
<I/σ(I)> | 16.7 | 2.31 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 20 * | citrate, ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | citrate | ||
2 | 1 | 1 | ammonium phosphate | ||
3 | 1 | 2 | citrate | ||
4 | 1 | 2 | ammonium phosphate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6-8 (mg/ml) | |
2 | 1 | drop | citrate | 100 (mM) | pH5.6 |
3 | 1 | drop | ammmonium dihydrogen phosphate | 1.0 (M) | |
4 | 1 | drop | xylitol | 0.3 (%) | |
5 | 1 | reservoir | citrate | 100 (mM) | pH5.6 |
6 | 1 | reservoir | ammmonium dihydrogen phosphate | 1.0 (M) | |
7 | 1 | reservoir | 400 (mM) |