1J19
Crystal structure of the radxin FERM domain complexed with the ICAM-2 cytoplasmic peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 100.440, 100.440, 99.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 * - 2.400 |
| R-factor | 0.229 * |
| Rwork | 0.228 |
| R-free | 0.24000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gc7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.350 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 * | 0.137 |
| Total number of observations | 599708 * | |
| Number of reflections | 23153 | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 4 * | PEG6000, MES, NaCl, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein/peptide solution | 0.27 (mM) | |
| 2 | 1 | 2 | MES/Na | 50 (mM) | pH6.0 |
| 3 | 1 | 2 | PEG6000 | 2 (%) | |
| 4 | 1 | 2 | 130 (mM) | ||
| 5 | 1 | 2 | dithiothreitol | 0.4 (mM) |
