1J19

Crystal structure of the radxin FERM domain complexed with the ICAM-2 cytoplasmic peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	100.440, 100.440, 99.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 * - 2.400
R-factor	0.229 *
Rwork	0.228
R-free	0.24000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gc7
RMSD bond length	0.008
RMSD bond angle	1.350 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.071 *	0.137
Total number of observations	599708 *
Number of reflections	23153
<I/σ(I)>		5.2
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	4 *	PEG6000, MES, NaCl, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein/peptide solution	0.27 (mM)
2	1	2	MES/Na	50 (mM)	pH6.0
3	1	2	PEG6000	2 (%)
4	1	2		130 (mM)
5	1	2	dithiothreitol	0.4 (mM)