1J0X
Crystal structure of the rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.700, 98.500, 183.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.930 - 2.400 |
| Rwork | 0.203 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Dimer of PDB ENTRY 1GD1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.440 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.930 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.062 * | 0.227 * |
| Total number of observations | 194719 * | |
| Number of reflections | 56521 | |
| Completeness [%] | 96.6 | 86.1 |
| Redundancy | 3.4 * | 3.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 3350, HEPES, K3-citrate, microseeding, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 16 (%(w/v)) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH8.0 |
| 3 | 1 | reservoir | potassium citrate | 0.2 (M) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
