1J0N

Crystal Structure of Bacillus sp. GL1 Xanthan Lyase that Acts on Side Chains of Xanthan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	2002-06-14
Detector	SIEMENS HI-STAR
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	54.357, 91.305, 159.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.400
R-factor	0.169
Rwork	0.169
R-free	0.24200 *
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.310 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASES
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.300	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.091 *	0.376 *
Total number of observations	55703 *
Number of reflections	28677	2308 *
Completeness [%]	89.9	53.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	PEG 4000, ammonium formate, sodium bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.18 (mg/ml)
2	1	reservoir	PEG4000	23 (%)
3	1	reservoir	ammonium formate	0.2 (M)
4	1	reservoir	sodium bicine	0.1 (M)	pH9.0