1J0A
Crystal Structure Analysis of the ACC deaminase homologue
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792, 0.9795, 0.9640 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 122.340, 122.340, 115.031 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.500 |
| Rwork | 0.201 |
| R-free | 0.26950 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 * | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.285 |
| Total number of observations | 34161 * | |
| Number of reflections | 2668 * | 282 * |
| <I/σ(I)> | 7.9 | 2.9 |
| Completeness [%] | 98.6 | 98.6 * |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 * | ammonium sulfate, 2-propanol, phosphate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | sodium potassium phosphate | 0.1 (M) | pH6.2-7.0 |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | 2-propanol | 3-8 (%(v/v)) |
