1IZC
Crystal Structure Analysis of Macrophomate synthase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 106.678, 106.678, 120.755 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.1777 |
Rwork | 0.176 |
R-free | 0.20300 * |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 22.978 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.081 | 0.315 |
Number of reflections | 41139 | |
<I/σ(I)> | 6.5 | 2.5 |
Completeness [%] | 91.3 | 61 |
Redundancy | 6.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |