1IZ5

Pyrococcus furiosus PCNA mutant (Met73Leu, Asp143Ala, Asp147Ala): orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-13
Detector	RIGAKU RAXIS V
Wavelength(s)	0.836
Spacegroup name	P 21 21 21
Unit cell lengths	64.289, 85.348, 118.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.800
Rwork	0.273
R-free	0.29750 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ge8
RMSD bond length	0.006
RMSD bond angle	1.198
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.086	0.602
Total number of observations	214024 *
Number of reflections	60694
<I/σ(I)>	4.7	1.4
Completeness [%]	99.6	98.8
Redundancy	3.5	6.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	20 *	ammonium sulfate, 2-methyl-2,4-pentanediol, sodium citrate, glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	100 (mM)	pH5.5
2	1	reservoir	ammonium sulfate	2.6 (M)
3	1	reservoir	MPD	11 (%(v/v))
4	1	reservoir	glycerol	4 (%(v/v))
5	1	reservoir	D143A/D147A