1IYX

Crystal structure of enolase from Enterococcus hirae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-25
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	153.510, 153.510, 90.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.246
Rwork	0.175
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ebh
RMSD bond length	0.006
RMSD bond angle	1.220 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.093	0.342
Number of reflections	30711
<I/σ(I)>		2.2
Completeness [%]	100.0 *	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	5 *	PEG4000, lithium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	100 (mM)	pH7.5
2	1	reservoir	PEG4000	25-30 (%)
3	1	reservoir		0.2 (M)
4	1	drop	protein	5-8 (mg/ml)