1IY9
Crystal structure of spermidine synthase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2001-11-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418, 1.5621, 1.7321 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.100, 105.800, 121.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.300 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 43983 | |
| Completeness [%] | 92.0 | 75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | PEG, salt, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
