1IY1

Crystal structure of the FtsH ATPase domain with ADP from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-06-06
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	73.875, 73.875, 84.611
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 2.800
Rwork	0.207
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.051 *	0.157 *
Number of reflections	6399
Completeness [%]	98.8	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	Ammonium sulfate, Tris-HCl, ADP, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH8.0
2	1	reservoir	ammonium sulfate	1.6 (M)
3	1	reservoir	glycerol	20 (%(v/v))
4	1	drop	AMP-PNP	100 (mM)
5	1	drop		40 (mM)